Francesco I. de' Medici (1541-1587), Grand Duke of Tuscany

Johanna of Austria (1547-1578), youngest daughter of Emperor Ferdinand I.

Vincenzo I. Gonzaga (1562-1612), Duke of Mantua; marriage on 29. April 1584

1. her son Francesco IV., Duke of Mantua, born on 7. May 1586, deceased on 22. December 1612, since 1608 married to Margarete (1589-1655), daughter of Duke Charles Emanuel I. of Savoy (1562-1630) and of Catalina Micaela of Spain (1567-1597); three children, of whom only the daughter Maria (1609-1660) survived childhood
2. her son Ferdinand, born on 26. April 1587, deceased on 29. October 1626; Cardinal from 1607 to 1612, since 1612 Duke of Mantua; his first spouse was Camilla Reticina, they married in 1615 and were divorced in 1616; his second spouse was Caterina de' Medici (1593-1629), they married in 1617; she was a daughter of Grand Duke Ferdinand I. of Tuscany; no children
3. her son Guglielmo Domenico, born on 4. August 1589, deceased on 13. May 1591
4. her daughter Margherita, born on 2. October 1591, deceased on 7. February 1632; since 24. April 1606 married to Duke Henry II. of Lorraine (1563-1624); four daughters
5. her son Vincenzo II., born on 8. February 1594, deceased on 25. December 1627; Cardinal from 1615 to 1616; since 1626 Duke of Mantua; since 1616 married to Isabella Gonzaga, a daughter of Alfonso Gonzaga, Margrave of Novellara; no children
6. her daughter Eleonora, born on 23. September 1598, deceased on 27. June 1655; on the 2. February 1622 she became the second wife of Emperor Ferdinand II. (1578-1637); no children