Cosimo I. de' Medici (1519-1574), Grand Duke of Tuscany

Eleonora of Toledo (1522-1562)

1. Johanna (or Giovanna) of Austria (1547-1578), the youngest daughter of Emperor Ferdinand I. († 1564) and Anna of Hungary and Bohemia († 1547); the wedding of Johanna and Francesco was celebrated in Florence on 15. December 1565
2. Bianca Capello or Bianca Cappello (1548-1587); initially his mistress; the wedding of Bianca and Francesco took place on 5. June 1578

from his first marriage, see: Johanna (or Giovanna) of Austria

from his relationship and second marriage with Bianca Capello (or Cappello): his son Antonio, born on 29. August 1576, deceased on 2. May 1621

• Bianca Capello (1548-1587): The Rise of a Mistress to a Legitimate Spouse, pp. 358-366, in: Maike Vogt-Luerssen: Frauen in der Renaissance – 30 Einzelschicksale (in German)
• Article: The True Faces of the Daughters and Sons of Cosimo I de' Medici